Pettenkofer Test. Pettenkofer’s Test For The Bile Acids To a fluid containing either or both bile acids or any solution of cholic acid add some cane sugar and then slowly drop by drop strong sulphuric acid The solution turns to a cherryred and then changes to purple.
In the meantime he studied at Würzberg devising the test for bile acid that bears his name Pettenkofer also started research into meat juices which inspired Liebig to investigate them and produce his extract of meat Despite Liebig’s unwillingness to see him go Pettenkofer eventually left Giessen to seek betterpaid employment in Munich.
Bile acids: analysis in biological fluids and tissues
PDF fileby his discovery of creatinin in urine and of the familiar test for bile acids which is so well known ” a Pettenkofer‘s s reaction” On his return to Munich the University authorities recommended to the Government that a Professorship of Physiological Chemistry should be founded and conferred on Pettenkofer This recommendation was however set aside and he had to content himself with.
Pettenkofer test definition of Pettenkofer test by
The Pettenkofer (31) test is probably the most sensitive reaction known at present for the bile acids As such it offers the best basis for a method which can be readily adapted for use with smal1 amounts of blood.
Pettenkofer, Max Josef von Encyclopedia.com
in the Pettenkofer test the Bile salt will react with sulfuric acid and sugar form the development of the hydroxymethylenefurfural to a furfural derivative which color is a progressive form red solution It is form under the influence of the reaction the speed of of sugar that dehydrated with.
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Test for Bile Acids Pettenkofer s test for sucrose Materials concentrated H2SO4 and 0 5ml sucrose Procedure A concentrated sulphuric acid was slowly added to the side of a test tube containing 05ml of sucrose (5% solution to 2ml of diluted bile acid) The color reaction at the point of contact was observed Result (+) Formation of red color at the point of contact.